It sounds like you still have the Trusted_Connection=True in your web.config ConnectionString.
If you have a remote database, you will need to remove the Trusted_Connection=True and put in User Id=PortalUser;Password=PortalUser and add the correct database name.
<appSettings>
<add key="ConnectionString" value="server=remoteserver;User Id=PortalUser;Password=PortalUser;database=YourDBName" />
Trusted_Connection works when the Database server is on the same machine as the Web Server.
Change your connection string and we can go from there.
Ben Miller
Microsoft MVP Lead
ASP.NET/XML/Web Services/E-Business Servers
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